National Center for Biotechnology InformationMethod developments to extract proteins from oil palm
Mesocarp preparation. To prepare high quality proteins from E. guineensis var. Tenera, a modification of the methods of Wang and co-workers (Wang et al. 2003, 2006; Gorg et
A histological study of oil palm (Elaeis guineensis) endosperm during seed development. J. Oil Palm Res., 27(2): 107-112. 2015: 134: Lau, B Y C; Deb-Choudhury, S; Morton, J D; Dyer, J M ; Ramli,
Method developments to extract proteins from oil palm
The ideal method was successfully used to establish the 2DE chromoplast proteome maps of low and high oleic acid mesocarps of oil palm. Further nanoLC-MS/MS analysis of the
An optimal procedure for extraction of proteins from oil palm root and leaf tissues was developed through evaluations and modifications of three existing methods.
MendeleyMethod developments to extract proteins from oil palm
(2015) Lau et al. SpringerPlus. Proteins from the plant chromoplast are essential for many physiological processes such as fatty acid biosynthesis. Different protein extraction methods were
S; Dyer, J M and Ramli, U S (2015). Method developments to extract proteins from oil palm chromoplast for proteomic analysis. Springerplus, 4: 791. Figure 2. Chromatograms of peptides
Efficiency of protein extraction from palm
Different protein extraction methods were tested to find the most robust method to obtain oil palm chromoplast proteins for mass spectrometry analysis. Initially, two different solvents were
Missing or different intensity spots are highlighted in dotted circles from publication: Method developments to extract proteins from oil palm chromoplast for proteomic analysis Proteins from
Effect of CDE in enhancing the number of protein spots in 2D
Selected protein spots for comparative analysis using PDQuest are indicated from publication: Method developments to extract proteins from oil palm chromoplast for proteomic analysis
Agrawal GK, Bourguignon J, Rolland N, Ephritikhine G, Ferro M, Jaquinod M, Alexiou KG, Chardot T, Chakraborty N, Jolivet P, Doonan JH, Rakwal R (2010) Plant organelle proteomics: collaborating
Proteomics analysis on lipid metabolism in Elaeis
Proteome data was obtained from the fruit mesocarps of the two oil palm species, namely, the African Elaeis guineensis (commercial tenera or commonly known as D x P and
PubMed:Effects of the Fruit Ripening Stage on Antioxidant Capacity, Total Phenolics, and Polyphenolic Composition of Crude Palm Oil from Interspecific Hybrid Elaeis oleifera Elaeis
PlusMethod developments to extract proteins from oil palm
Lau et al. SpringerPlus DOI 10.1186/s40064-015-1576-4 RESEARCH Method developments to extract proteins from oil palm chromoplast for proteomic analysis Benjamin Yii Chung Lau1,2,3*,
Our results revealed protein changes in early oil palm fruit development provide valuable information in the understanding of fruit growth and metabolism during early stages that
Effects of solvent washes on 2DE protein profiles
Missing or different intensity protein spots are highlighted in dotted circles from publication: Method developments to extract proteins from oil palm chromoplast for proteomic analysis Proteins
Method developments to extract oil palm chromoplast proteins for proteomic analysis.脗 SpringerPlus, 4(1): 791. 2015 147
- What is the primitive method of plant ECM protein isolation?
- Perhaps the primitive method of plant ECM protein isolation involves the direct extraction in solution containing CaCl 2 gradients and plasmolysis of plasma membrane to shrink ECM [ 11, 12 ].
- What is the protocol for ECM extraction & protein extraction?
- The protocol for ECM isolation, protein extraction , and mass spectrometry coupled gel-based analysis described in this chapter is simple, reproducible, robust, and easy to carry out, that yield intact and pure ECM fraction and ECM proteins.
- What are the caveats of plant ECM protein extraction & isolation?
- Extraction and isolation of plant ECM proteins features key caveats due to the lack of adjoining membrane, the presence of a polysaccharide–protein network that traps contaminants, and the existence of high phenolic content.
- How are ECM proteins extracted?
- The major steps involved mechanical disruption, density-based gradient centrifugation and series of stringent washes to remove contaminating proteins and extraction of ECM proteins with commonly used wall protein extractants, CaCl 2 (Subheadings 3.1 and 3.3 ). Rigorous purity assessment is performed at organellar and protein levels.
- How are ionically bound ECM proteins extracted?
- Ionically bound ECM proteins were eluted by incubating cultured cells in 1 M NaCl buffer followed by homogenization, vacuum infiltration, and extraction. 0.2 M CaCl 2 and potassium phosphates were used to extract tightly ionically bound (fraction 2) ECM proteins.
- How to separate ECM protein from insoluble fraction?
- Shake the ECM pellet with extraction buffer on a shaking platform for 2 h at 4 °C to remove any further contaminants and to release ECM proteins ( see Note 16 ). 4. Following centrifugation at10,000 × g for 10 min at 4 °C, the ECM proteins gets separated from the insoluble fraction ( see Note 17 ).
