Surface structure and properties of plant seed oil bodies
It is concluded that both oleosins and PL are required to stabilize the oil bodies and that oleosins prevent oil bodies from coalescing by providing steric hindrance. A structural model of an oil body
May 1, 1992Figure S. Stability of oil bodies reconstituted from TAG, PL, and/or maize oleosins . A suspension of the reconstituted oil bodies of 1.4 ml was placed in a cuvette, and the relative
The structural organization of seed oil bodies could explain the
The structural organization of seed oil bodies could explain the contrasted oil extractability observed in two rapeseed genotypes Planta. 2015 Jul These organelles consist of a core of neutral lipids
ABUNDANT OIL BODIES IN SEED CELLS: DYNAMIC FUNCTIONS OF MEMBRANE PROTEINS. Oil body formation begins on the endoplasmic reticulum (ER) membrane (Fig. 2A;
Structure and functions of oleosomes (oil bodies) ScienceDirect
On the other hand, the oil in eukaryotes is already in the form of emulsified droplets, stabilized by a unique protein/phospholipid membrane. Those natural oil droplets that can be
The protein, phospholipid and sterol composition of the oil body surface from the seeds of two rapeseed genotypes was compared in order to explain their contrasted oil extractability. In the
Analytical Science JournalsCrop seed oil bodies: From challenges in protein identification
Oleaginous seeds store lipids in specialized structures called oil bodies (OBs). These organelles consist of a core of neutral lipids bound by proteins embedded in a phospholipid
OBs are organelles that store TAG in the cytoplasm and play a vital role in seed development and germination [6,20]. Prior studies have noted the importance of the relationship between OB and OC
Deciphering the structural organization of the oil bodies
To confirm some of these observations obtained from these two most contrasted accessions with respect of their oil yield, another study was carried out in replicate at pH 7.7 and
Figure 7. The intron position in each of the three oleosin isoforms. Chen MCM, Peng C-C, Tzen JTC (2002) Gene family of oleosin isoforms and their structural stabilization in sesame seed oil
Steroleosin, a Sterol-Binding Dehydrogenase in Seed Oil Bodies
Vegetable cooking oils are triacylglycerols (TAGs) extracted from various plant seeds. The storage of TAGs is confined to the discrete spherical organelles called oil bodies (Yatsu and Jacks, 1972;
The structural and physicochemical characteristics of oil bodies from hemp seeds were explored in this study. Oil bodies from several plant-based sources have been previously studied; however
Fatty acid composition (%) of lipids in hemp seed oil bodies,
The structural and physicochemical characteristics of oil bodies from hemp seeds were explored in this study. Oil bodies from several plant-based sources have been previously studied; however
The structural and physicochemical characteristics of oil bodies from hemp seeds were explored in this study. Oil bodies from several plant-based sources have been previously studied; however
Oil bodies: An insight on their microstructure ScienceDirect
During recent years numerous research articles have appeared concerning the extraction, isolation and characterization of oil bodies from different sources (Acevedo et al.,
Microstructures of SBOBs, PNOBs and SFOBs are shown in Figure 1S. After being extracted by the buffer solution, the OBs can disperse in aqueous medium, and the structure of which remain
- Do seed oil bodies contain lipid particles?
- A structural model of an oil body is presented. The current findings on seed oil bodies could be extended to the intracellular storage lipid particles present in diverse organisms. Storage triacylglycerols (TAG) in plant seeds are present in small discrete intracellular organelles called oil bodies.
- How are oil-bodies reconstituted?
- Reconstitution oil-bodies. Oil-bodies were reconstituted by mixing either pure triolein or pure rapeseed oil (neutral oils only) with purified phosphatidylcholine (Sigma P1013) and/or the oilbody membrane apolipoprotein in the proportion 100 parts oil: 10 parts apolipoprotein: 2-5 parts phospholipid (w/w).
- How are purified seed oil-bodies isolated?
- A simple, rapid procedure for the isolation of purified seed oil-bodies is reported. The oil-bodies were collected by two floatation steps following centrifugation of tissue homogenates. The oil-bodies from a variety of oilseed species contained ca 80% triacylglycerol, 15% apolipoprotein and 2% phospholipid.
- What is the role of apolipoproteins in seed oilbodies?
- The role of the apolipoproteins in seed oilbodies is discussed. INTRODUCTION Oilseed crops have tremendous potential as sources of both edible oils and renewable oil-based raw materials for the chemical and pharmaceutical industries. Many oilseed crops are also good sources of high quality protein for both livestock and human consumption.
- How do we reconstitute oil bodies from their constituents?
- Attempts were made to reconstitute oil bodies from their constituents. TAG, either extracted from oil bodies or of a 1:2 molar mixture of triolein and trilinolein, in a dilute buffer were sonicated to produce droplets of sizes similar to those of oil bodies; these droplets were unstable and coalesced rapidly.
- What is seed storage oil?
- Seed storage oils are synthesized during seed development [2, 3] either in the embryonic tissue, as in rapeseed, sunflower seed or soybeans, or in another tissue as in castor bean (endosperm). In all cases, the seed oil is eventually deposited into oilbodies, which generally range from 0.4 to 1.5 um average diameter in the mature seed tissue.
