• aqueous enzymatic extraction of coconut oil in Durban
  • aqueous enzymatic extraction of coconut oil in Durban
  • aqueous enzymatic extraction of coconut oil in Durban
  • aqueous enzymatic extraction of coconut oil in Durban
  • Which enzymes are used to extract coconut oil?
  • Aqueous extraction of coconut oil with various enzymes was investigated. Several enzyme preparations (cellulase, polygalacturonase, protease, and α-amylase) were used at different concentrations, pH, and temperature values to enhance oil extraction. After the oil had been released by the enzyme reaction, it was separated by centrifugation.
  • What is aqueous enzymatic extraction?
  • The adverse environmental impacts of conventional oil processing technologies have forced the development of new green technologies. The aqueous enzymatic extraction (AEE) destroys the oilseed through enzymatic hydrolysis and separates the oil and hydrophilic components with water, to achieve simultaneous separation of oil and protein etc.
  • What is aqueous extraction?
  • This method is known as aqueous extraction (AE) to separate oil by different affinities of oil and non-oil components, and difference of oil and water gravity. In 1959, Subrahmanyan et al. (Subrahmanyan et al., 1959) optimized AE, which resulted in the peanut oil yield of only 30%, and the separated protein product contained 12% oil.
  • How is oil extracted?
  • After the oil had been released by the enzyme reaction, it was separated by centrifugation. The results showed that an enzyme mixture at 1% (w/w) each of cellulase, α-amylase, polygalacturonase, and protease at pH 7.0 and an extraction temperature of 60°C represented the most effective extraction conditions with an oil yield of 73.8%.
  • How did AE improve the extraction conditions of peanut oil & protein?
  • In 1972, Rhee et al. (Rhee et al., 1972) optimized the extraction conditions of peanut oil and protein by AE, and began to study the effect of the crushed particle size of oilseed on the oil yield. AE does not use solvents and desolvation treatment, but the problem of low oil yield has not been solved.
  • Can enzymes be used to extract oil by AEE?
  • Two barriers (oilseed cell wall and oil body interfacial membrane) need to be overcome to extract oil by AEE. The selection, application, and influencing factors of enzymes in AEE were discussed. UAEE, MAEE, UMAEE, and PAEE can increase the oil yield and shorten the extraction time. Immobilized enzymes can increase the reusability of enzymes.