SCISPACEDevelopment of a method for DNA extraction from oil
eiciency. Progress would require an eicient, low cost and high-throughput deoxyribonucleic acid (DNA) extraction method adapted for oil palm. A method for genomic DNA extraction from oil
Development of a Method for DNA Extraction From Oil Palm Leaves and the Effects of pH and Ionic Strength on Nucleic Acid Quantification Journal of Biological Methods doi
Development of a method for DNA extraction
A method for genomic DNA extraction from oil palm leaves that is simple and cost-effective was developed without the use of liquid nitrogen. A 260/280 ratios between 1.987 and 2.078 were obtained.
Table 1. Comparative assessment of the different protocols used to isolate DNA from oil palms. "Development of a method for DNA extraction from oil palm leaves and the effects of pH and ionic
EppendorfAutomated DNA Extraction from Oil Palm Leaves using
Fresh oil palm leaves (50 mg wet weight or 0 mg lyophilized tissue) are measured into each tube in 1.2 mL collection microtube racks (supplied with DNeasy 96 Plant Kit) containing one 3 mm
The protocol was based on Ihase et al. (2016) for the extraction of DNA from oil palm leaves with modifications. One hundred milligram of leaf sample was powderized using liquid nitrogen and
A simple and rapid protocol for isolation
Genomic DNA for most of the samples was extracted by a service provider at the Centre for Marker Discovery and Validation (CMDV), Malaysia whereas, about 20% of the DNA extraction were carried out
After assessing the purity and amplificability of the DNA extracted from olive, sunflower and palm oil with the methods A-H (Table 2), and considering the reduced oil volume required by
DNA EXTRACTION FROM MATURE OIL PALM LEAVES
The starting point for such applications is DNA extraction using young, immature leaves as required by common protocols. Since such leaves are sometimes not available or are difficult to obtain, a
Representative spectra comparing the improved method (new) to the original DNA extraction method (old). The spectra were recorded using a NanoDrop 2000 spectrophotometer and overlayed for
Automated, high-throughput DNA extraction protocol
The approach in this paper included design and development of DNA extraction protocol on disposable microfluidics device integrated with biosensor for automated, high
DNA analysis is a fast and economic tool to identify plant components in oils. Extraction and amplification of DNA by PCR was tested in olives, in milled seeds and in oils, to
Preservation of Oil Palm (Elaeis guineensis Jacq.) leaf tissues for DNA
This study attempts to investigate methods of preserving oil palm (Elaeis guineensis Jacq.) leaf tissue prior to DNA extraction. Oil palm was preserved using physical method by storing leaf
Methods DNA was extracted from the different tissues mentioned above using the modified DNA extraction method of Dellaporta et al. (1983). The tissues were collected separately from each of
Development of simple sequence repeat (SSR) markers for oil palm
Sep 30, 2007This study describes the application of a simple and effective method to isolate SSR markers from oil palm genomic sequences. A total of 12 informative SSR markers are described.
One leaf sample each of Chilean wine palm (Jubaea chilensis), oil palm (Elaeis guineensis), and jelly palm (Butia capitata) were obtained from the Palmengarten Frankfurt/Main, Germany. All leaf
- How to extract DNA from oil palm leaves without liquid nitrogen?
- Progress would require an efficient, low cost and high-throughput deoxyribonucleic acid (DNA) extraction method adapted for oil palm. A method for genomic DNA extraction from oil palm leaves that is simple and cost-effective was developed without the use of liquid nitrogen. A 260/280 ratios between 1.987 and 2.078 were obtained.
- How is DNA extracted from palm trees?
- Using the developed protocol, DNA was isolated from 10 palm trees comprising four pisifera, one tenera (T) and five dura palms. Eight microliters (8 µl) of each sample were loaded on a 0.8% agarose gel and the gel was stained with ethidium bromide. Total DNA from oil palm leaves of 93 trees extracted with the protocol.
- Can sterile sand be used to Grind Date palm leaves?
- Isolation of highly pure DNA is the prerequisite for PCR amplification and subsequent use such as DNA fingerprinting and sequencing of genes that have recently been developed for barcoding. To avoid problems related to the preservation and use of liquid nitrogen, we examined sterile sand for grinding the date palm leaves.
- What are the components of DNA extraction?
- The components of the DNA extraction buer used a previous protocol used for mature leaves of oil palm [ 6]. Mannitol, of Tris-HCl, EDTA and SDS are also dierent. The method reported [ 7, 8]. Liquid nitrogen, which is not always easy to obtain in most third Billotte et al. (Table 1). The cost of DNA extraction per sample
- Where is DNA extracted?
- Genomic DNA for most of the samples was extracted by a service provider at the Centre for Marker Discovery and Validation (CMDV), Malaysia whereas, about 20% of the DNA extraction were carried out in-house, using a modified CTAB method (Rahimah et al. 2006; Suzana et al. 2015).
- Can leaf sampling be used for DNA extraction?
- Regarding the leaf sampling for (spear) leaves can also be used for DNA extraction. concentration of the extracted DNA. Here, we determined the eect of metric absorbance of the extracted nucleic acids. The method reported as visible in Figure 1. The treatment of the nucleic acids with RNase was omitted for two reasons.
